醫(yī)學(xué)論文范文:Toll樣受體2對(duì)角質(zhì)形成細(xì)胞分泌IL8的影響

醫(yī)學(xué)論文范文:Toll樣受體2對(duì)角質(zhì)形成細(xì)胞分泌IL8的影響

【摘要】 目的:觀(guān)察白念珠菌刺激人角質(zhì)形成細(xì)胞后IL��8的變化以及Toll樣受體2(Toll�瞝ike receptor 2，TLR��2)對(duì)IL��8分泌的影響，探討角質(zhì)形成細(xì)胞在皮膚免疫系統(tǒng)中的作用。方法：白念珠菌分別與抗TLR��2抗體預(yù)處理前后的角質(zhì)形成細(xì)胞孵育，ELISA方法檢測(cè)不同時(shí)間培養(yǎng)上清液IL��8的水平。結(jié)果：白念珠菌可引起IL��8明顯升高(P<0.01)，3h開(kāi)始，至24h達(dá)到高峰;中和TLR��2受體后，雖然引起IL��8升高,但無(wú)統(tǒng)計(jì)學(xué)意義(P>0.05)。結(jié)論：白念珠菌可以刺激角質(zhì)形成細(xì)胞分泌IL��8，TLR��2在角質(zhì)形成細(xì)胞IL��8表達(dá)中的作用尚難確定。

【關(guān)鍵詞】 白念珠菌 Toll樣受體2 IL 8 角質(zhì)形成細(xì)胞 天然免疫

Effects of Toll�瞝ike Receptor��2 on Interleukin��8 Secretion of Keratinocytes HUANG Guang�瞨ong1, FAN Li1, DUAN Ying�瞔hun1, DUAN De�瞛ian2^ (1Department of Obstetrics and Gynecology,Renmin Hospital;2Department of Dermatovenereology,Taihe Hospital,Yunyang Medical College,Shiyan,Hubei 442000,China)

Abstract: Objective To observe the change of Interleukin��8(IL��8) in keratinocytes induced with Candida Albicans and the effect of Toll�瞝ike receptor��2(TLR��2) on IL��8 secretion,to explore the role of keratinocytes in skin immune system. Methods Candida Albicans were cultured on keratinocytes treated before and after anti�睺LR��2 monoantibody(mAb),respectively.The levels of IL��8 in culture supernatant were determined with ELISA at different time points. Results The IL��8 levels were significantly increased after induced with Candida Albicans(P<0.01),the significant increase was observed at 3 h and reached the peak at 24 h;the increase of IL��8 levels had no significant difference after TLR��2 was neutralized with anti�睺LR��2 mAb(P>0.05). Conclusion The secretion of IL��8 in keratinocytes could be induced with Candida albicans,the function of TLR��2 is uncertain in expression of IL��8 in keratinocytes醫(yī).學(xué)全.在.線(xiàn)網(wǎng)站quanxiangyun.cn.

Key words:Candida albicans;Toll�瞝ike receptor��2;Interleukin��8;Keratinocytes;Innate immunity

念珠菌在人體表面的分布廣泛而常見(jiàn)，而白念珠菌是引起念珠菌病的主要致病菌種。在去除表皮的實(shí)驗(yàn)說(shuō)明表皮角質(zhì)形成細(xì)胞在抵抗念珠菌感染中起重要作用。皮膚粘膜念珠菌病常表現(xiàn)為病原菌寄生部位的角層下膿皰或角層內(nèi)大量中性粒細(xì)胞聚集，但是否與細(xì)胞表達(dá)的趨化因子IL��8有關(guān)，目前還不太清楚[1]。Toll樣受體2(Toll�瞝ike receptor 2，TLR��2)是表達(dá)于角質(zhì)形成細(xì)胞并參與真菌產(chǎn)物識(shí)別的天然受體蛋白，其與IL��8的關(guān)系亦研究不多，故本研究旨在探討TLR��2對(duì)角質(zhì)形成細(xì)胞分泌IL��8的影響。

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